Available cartridges and reactions
How does Synple work?
Settings up a reaction on Synple 2
Comparison of Synple 1 vs manual synthesis

Available reaction classes
N-Heterocycle formation

- 10 different Heterocycle structures currently available
- Scale: up to 0.5 mmol
Advantages compared to classical batch chemistry are:
- Minimise user exposure to toxic tin reagents
- Multistep reactions completed at the touch of a button
- High reproducibility

What is in the heterocycle formation cartridges:

Reductive Amination

The cartridge contains all reagent for the condensation, reduction and purification steps of the process.
- Scale: up to 0.5 mmol
- Time-saving – reaction, work up and purification in a single process
- Automation of routine chemistry to allow more time for technically demanding reactions
- Generic methods, selected based on reaction partners, minimize need for reaction optimization.
What is in the reductive amination cartridges:

Mitsunobu Reaction

- Scale: up to 0.5 mmol
- Applicable to a range of pronucleophiles (phenols, phthalimides, tosylamides, tosylhydrazones).
- Only 5-10 working time required to obtain Mitsunobu product.
- No tedious removal PPh3O necessary.
What is in the Mitsunobu cartridges:

PROTAC formation

The cartridge contains all reagents for the linking the carbonyl group of a protein binder with the partial PROTAC and components for the purification steps of the process.
- Scale: up to 0.1 mmol

What is in the PROTAC cartridges:

Biotin Tags

Biotin Tags
The cartridge contains all reagents for the linking the compound of choise to a variety of biotin tags and also includes components for a simple purification step.
- Scale: up to 0.1 mmol

What is in the Biotin cartridges:
For Amine-Linker

For Amide-linker

Deoxyfluorination

Deoxyfluorination
Fluorinations are among the most interesting reaction in medicinal chemistry and mostly used for late stage functionalization.
The subgroup of Deoxyfluorination involves the reaction between an alcohol and a fluorinating agent to generate the corresponding fluorinated product.
Using the approach the Synple Chem synthesizer offers an easy and fast automated method for the deoxyfluorination of primary and secondary alcohols without the need of handling fluorinating agents.
- Scale: up to 0.2 mmol
What is in the Fluorination cartridges:

Boc Protection

Boc protection
The Synple Chem synthesizer offers an easy and fast automated method to Boc-protect
primary and secondary amines with less than 5 min working time and no left-over Boc-Anhydride.
- Scale: two sizes available; up to 0.5 mmol and up to 1.2 mmol
What is in the Boc protection cartridges:

Boc Deprotection

Boc deprotection
What is in the Boc deprotection cartridges:

Azide formation

Azide formation
Azide formation by diazo transfer of primary amines is a highly important reaction in organic chemistry and chemical biology. Azides have been widely utilized as the key partner in Cu-catalyzed azide-alkyne cycloaddition reactions for bioorthogonal click chemistry, peptide conjugation and polymerization processes. Azides also show good chemical stability so that they can serve as a protecting group for primary amines, in particular in the field of carbohydrate chemistry. Previous methods of converting amines into azides require fresh preparation of diazo transfer agents (not commercially available) using highly hazardous reagents, which has largely limited its broad adoption.
- Scale: up to 0.5 mmol
- Avoid handling azidation reagents
What is in the Azide cartridges:
