Available cartridges and reactions
How does Synple work?
Settings up a reaction on Synple 2
Comparison of Synple 1 vs manual synthesis
Available reaction classes
N-Heterocycle formation
Our first, currently available reaction class facilitates the generation of N-Heterocycles. These include morpholines, piperazines, oxazepanes, diazepanes and more complex spiro and bicyclic structures. A broad scope of structurally and electronically different aldehydes can be readily transformed to the N-heterocycle in a very easy manner following just a 10 min reaction setup.
Advantages compared to classical batch chemistry are:
- 10 different Heterocycle structures currently available
- Scale: up to 0.5 mmol
Advantages compared to classical batch chemistry are:
- Minimise user exposure to toxic tin reagents
- Multistep reactions completed at the touch of a button
- High reproducibility
What is in the heterocycle formation cartridges:
Reductive Amination
The second reaction class currently enables a reductive amination process. A larger number of complex amines can be generated easily from aldehydes or ketones and primary or secondary amines.
The cartridge contains all reagent for the condensation, reduction and purification steps of the process.
The cartridge contains all reagent for the condensation, reduction and purification steps of the process.
- Scale: up to 0.5 mmol
- Time-saving – reaction, work up and purification in a single process
- Automation of routine chemistry to allow more time for technically demanding reactions
- Generic methods, selected based on reaction partners, minimize need for reaction optimization.
What is in the reductive amination cartridges:
Mitsunobu Reaction
Since its discovery in 1967, Mitsunobu reaction has got a privileged role in organic synthesis and medicinal chemistry because of its scope, stereoselectivity and mild reaction conditions. Its ability of easily forming carbon-carbon bond through dehydrative coupling of a primary or secondary alcohol with a pronucleophile is sometimes counterbalanced by the formation of byproducts which can plague purification strategies. With the Synple machine Mitsunobu reaction can be run without these drawbacks.
- Scale: up to 0.5 mmol
- Applicable to a range of pronucleophiles (phenols, phthalimides, tosylamides, tosylhydrazones).
- Only 5-10 working time required to obtain Mitsunobu product.
- No tedious removal PPh3O necessary.
What is in the Mitsunobu cartridges:
PROTAC formation
The third reaction class enables the formation of PROTACs. Both VHL and CRBN Ligands are available with different lenghts of PEG linkers.
The cartridge contains all reagents for the linking the carbonyl group of a protein binder with the partial PROTAC and components for the purification steps of the process.
The cartridge contains all reagents for the linking the carbonyl group of a protein binder with the partial PROTAC and components for the purification steps of the process.
- Scale: up to 0.1 mmol
What is in the PROTAC cartridges:
Biotin Tags
Biotin Tags
The fourth reaction class enables the attachemnt of Biotin tags to amines and aldehydes/ketones. Different lenghts of PEG linkers are available.
The cartridge contains all reagents for the linking the compound of choise to a variety of biotin tags and also includes components for a simple purification step.
The cartridge contains all reagents for the linking the compound of choise to a variety of biotin tags and also includes components for a simple purification step.
- Scale: up to 0.1 mmol
What is in the Biotin cartridges:
For Amine-Linker
For Amide-linker
Deoxyfluorination
Deoxyfluorination
Fluorinations are among the most interesting reaction in medicinal chemistry and mostly used for late stage functionalization.
The subgroup of Deoxyfluorination involves the reaction between an alcohol and a fluorinating agent to generate the corresponding fluorinated product.
Using the approach the Synple Chem synthesizer offers an easy and fast automated method for the deoxyfluorination of primary and secondary alcohols without the need of handling fluorinating agents.
- Scale: up to 0.2 mmol
What is in the Fluorination cartridges:
Boc Protection
Boc protection
N-Boc protections are one of the most widely used protection groups used in synthetic chemistry.
The Synple Chem synthesizer offers an easy and fast automated method to Boc-protect
primary and secondary amines with less than 5 min working time and no left-over Boc-Anhydride.
The Synple Chem synthesizer offers an easy and fast automated method to Boc-protect
primary and secondary amines with less than 5 min working time and no left-over Boc-Anhydride.
- Scale: two sizes available; up to 0.5 mmol and up to 1.2 mmol
What is in the Boc protection cartridges:
Boc Deprotection
Boc deprotection
What is in the Boc deprotection cartridges:
Azide formation
Azide formation
Azide formation by diazo transfer of primary amines is a highly important reaction in organic chemistry and chemical biology. Azides have been widely utilized as the key partner in Cu-catalyzed azide-alkyne cycloaddition reactions for bioorthogonal click chemistry, peptide conjugation and polymerization processes. Azides also show good chemical stability so that they can serve as a protecting group for primary amines, in particular in the field of carbohydrate chemistry. Previous methods of converting amines into azides require fresh preparation of diazo transfer agents (not commercially available) using highly hazardous reagents, which has largely limited its broad adoption.
- Scale: up to 0.5 mmol
- Avoid handling azidation reagents
What is in the Azide cartridges:
Details about two reaction classes can be found in our recent poster:
Are you looking for another chemistry application? Contact us!
You want to know more?
Frequently Asked Questions
Who is Synple Chem?
Snyple Chem was founded in 2016 as a Spin-off from ETH Zürich. Our mission is to provide easy to use, safe, efficiency-enhancing solutions that represent the future of organic synthesis.
How many products are on the market?
Now, alongside the Synple 1 and 2, we have our latest addition - the Synple Unity, a flexible parallel synthesiser.
Can I put my own materials in the cartridges?
No, the cartridges come ready filled with all the reagents and materials required for the reaction, work-up and purification. We can, however, provide custom cartridges for users under certain circumstances.
What solvents are compatible with the machine?
The machine itself is tolerant of almost all solvents. The limitation are only related to what is tolerated in terms of the chemistry by each reaction class. More information can be found in our application notes.
How does the machine handle poorly soluble starting materials?
In many cases, co-solvents can be added to help solubiulise poorly soluble starting materials.
How are lower reacting starting materials handled?
We provide a scope for each reaction that indicates what is fast reacting and slow reacting. For slower reacting materials we recommend increasing the default reaction time.
Can I run more than one reaction at the same time?
With the Synple 1 and Synple 2, only one reaction can be run at a time. The Synple Unity system allows you to build a parallel synthesis system to suit your own needs.
How much maintenance is required?
The machine requires very little maintenance and most of the components, e.g. the lines, connections, vial, are all standard. We are also able to offer service and maintenance contracts.
Do you have products on the market?
Yes! Our Synple 1 machines have been installed with customers since May 2018 and our new Synple 2s have been in use since October 2019.
In which countries is Synple available?
At the moment we sell directly across Europe and the UK. We can also sell direct in the US or alternatively use a distributor. In addition, we are in discussions with several distributors across Asia.
What is the lead time for the machine and cartridges?
The average lead time for the machine is four weeks. Cartridges can be delivered within the week.
Can my starting material be dissolved in a broad range of solvents?
This depends on the reaction class and what is tolerated by each particular reaction. In many cases co-solvents can be used to help solubilise the starting material.
Does the machine allow for reaction monitoring?
Each method has been pre-optimised to give the best yield and purity across a broad substrate scope. If optimisation is required then we would recommend skipping the purification step and checking the product at the end of the run. If it has not gone to completion, the reaction can be run again.
Can the machine be connected to analytical or purification systems?
This is something we are actively exploring.
How long does a run take?
This is dependant on the reaction class. Each run time includes the reaction, work-up and purification time.